PLATFORM FOR THE ISOLATION OF ORGANELLES IN LEUKEMIA CELLS TO ASSESS FUNCTIONAL MODULATIONS LINKED TO PATHOLOGY OR CHEMOTHERAPEUTIC TREATMENT (AML/MDS/CMML)
- Preclinical development
- AML
- MDS
- CMML
- Targeted therapy
- Immunotherapy
- Chemotherapy
- Preclinical research (TRL 4-5)
Isolation of cellular organelles to assess functional modulations linked to pathology or chemotherapeutic treatment.
The lysosomes and mitochondria that we purify retain their integrity and functionality intact.
Description
Scope of research activities
Preclinical research using subcellular fractions of myeloid leukemia cells:
- Subcellular localization
- Functional studies
Conduct of studies
Steps:
- Analysis of study strategy
- Study design
- Drafting of study plans
- Organization, implementation and conduct of studies
- Analysis and communication of results
Research infrastructure
Experimental and analytical platforms:
- Nitrogen cavitation
- Gradient ultracentrifugation
- Flow cytometry
- Western blot
Cell models: (human and murine)
- Primary cells
- Tumor cells
- Cell lines
Technical personnel:
- 1 engineer
Specifications
The platform
Isolation of cellular organelles for the evaluation of functional modulations linked to a pathology or chemotherapeutic treatment.
The studies
Subcellular localization:
- Nucleus/Cytoplasm/Membrane
- Lysosome
- Mitochondria
- Autophagosomes
Functional studies:
- Mitochondria (Permeabilization; Loss of potential)
- Lysosomes (Intra-lysosomal pH analysis)
Terms
OPALE entity
C3M (UMR-1065)
Team leader
Contact
Dr. Sandrine PalcyBusiness Development Director
Other offers
PLATFORM FOR SCREENING MOLECULES ABLE OF REPROGRAMMING PRIMARY HUMAN MACROPHAGES (AML/Myelofibrosis/MDS/CMML)
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IN CELLULO IDENTIFICATION OR VALIDATION OF THERAPEUTIC TARGETS IN MYELOID LEUKEMIA USING CLICK CHEMISTRY TECHNOLOGY TO DEVELOP NEW DIAGNOSTIC METHODS OR TARGETED THERAPIES