STUDIES OF THE MECHANISM OF HEMATOPOIETIC STEM CELL TRANSFORMATION IN ACUTE MYELOID LEUKEMIA AND CLONAL SELECTION MODELS
- Target discovery
- AML
- Targeted therapy
- Alternative therapy
- Basic research (TRL 1-3)
Study of the microenvironment’s role (i.e. stromal cells, marrow plasma composition) in the transformation of hematopoietic stem cells in acute myeloid leukemia (AML) to understand the action of anti-leukemic drugs on clonal selection in AML. Comparative study with somatic genetic rescue phenomena in constitutional hematopoietic pathologies.
Expertise in the dialogue between hematopoietic cells and stromal cells, identification of microenvironment targets. Perspectives for combined action on leukemia cells and the pathological bone marrow microenvironment.
Description
Scope of research activities
Mechanistic studies of hematopoietic stem cells transformation in AML.
Conduct of studies
Steps:
- Evaluation of questions and objectives
- Design of studies and experimental plans
- Experimental and analytical implementation
- Valorization of results
Research infrastructure
Experimental and analytical platforms:
- Cell culture, co-culture
- Cytometry
- Microscopy/imaging
- Genomics, transcriptomics, single cell RNAseq
- Cytokine assays, protein analysis
Models:
- Primary hematopoietic and mesenchymal cells from healthy individuals
- Primary hematopoietic and mesenchymal cells from AML patients
- Stromal and leukemic cell lines
Technical personnel:
- Technicians / Engineers
Specifications
Our approach
Strategy based on our knowledge of the clonal architecture of AML and our tools for understanding the initiation and evolution of leukemic and pre-leukemic clones, integrating parameters of the bone marrow microenvironment as well as parameters dependent on predisposing situations. Study of the dialogue between hematopoietic cells and the microenvironment depending on pathology.
Studies
- Genomic analysis (bulk and single cell)
- Transcriptomic analysis (bulk and single cell)
- Hematopoietic functional analysis
Terms
OPALE entity
CRSA (UMR-938)
![François Delhommeau](/media/utilisateur/images/20240216125857_14_U938_F-Delhommeau.jpeg)
Head of Entity
Contact
![Sandrine Palcy](/media/utilisateur/images/20231114213851_Sandrine_Palcy.jpg)
Business Development Director